百合疫病病原鉴定及其PCR检测
Identification and PCR Detection of Phytophthora Blight of Lily
杨秀梅 1瞿素萍 1王丽花 1刘晓侠 2彭绿春 1王继华1
作者信息
- 1. 云南省农业科学院花卉研究所,云南省花卉育种重点实验室,昆明,650205
- 2. 浙江嘉兴学院生化学院,浙江嘉兴,314001
- 折叠
摘要
对从百合疫病病株上分离得到的病原菌进行了形态特征观察、致病性测定及核糖体DNA-ITS序列分析.结果表明,该菌为疫霉属真菌,与GenBank中烟草疫霉ITS序列的同源性为98%～99%.结合病原菌PCR检测结果,进一步证实其为烟草疫霉菌(Phytophthora nicotianae).这是烟草疫霉菌侵染云 南切花百合的首次报道.
Abstract
The pathogen causing lily Phytophthora blight was isolated from diseased plants with typical symptoms on basal stems. The fungi was identified as Phytophthora nicotianae according to morphological characteristics, pathogenicity and the sequence of ribosomal DNA-ITS. The sequence of ribosomal DNA-ITS of isolate PI was showed a sequence identity of 98% - 99% with Phytophthora nicotianae published in GenBank. PCR detection using P. Nicotianae - specific primers indicated that the pathogen was P. Nicotianae. This is the first report of Phytophthora blight of lily in Yunnan.
关键词
百合/疫病/病原鉴定/PCR检测Key words
lily/Phytophthora blight/pathogen identification/PCR detection引用本文复制引用
基金项目
国家科技支撑计划(2007BAD45B01)
国家科技支撑计划(2007BAD45B04)
云南省自然科学基金(2007C121M)
出版年
2011
国家科技期刊平台
NSTL
维普
万方数据