Somatic Embryogenesis and Plant Regeneration in Hippeastrum hybridum
An effective micropropagation system via callus-mediated（indirect）somatic embryogenesis was established,using in vitro bulblets as explants in Hippeastrum hybridum.The results showed that 2,4-D（0.5 mg·L1）and 6-BA（0.5 mg·L-1）had important role in inducing embryogenic callus from in vitro bulblets and in multiplication of callus during sub-cultures.The percentage of friable callus formation was 83.3%in primary culture.The 6-BA（2.5-5.0 mg·L1）had a notable effect on development of somatic embryos.The rate of callus with more than twenty somatic embryos was about 50%for 50 days.Development of somatic embryos had no difference on the same 6-BA media supplemented with different concentrations of NAA.
NETL
NSTL
万方数据
维普
