Cloning and Characterization of Dehydrins Gene SlDHN2b in Tomato
In present study,a cDNA clone,designated SlDHN2b,was isolated from tomato by RT-PCR. The ORF of SlDHN2b was 1 140 bp and contained 380 amino acid residues. Protein sequence analysis showed that the SlDHN2b was high hydrophilic. Real-time PCR analysis indicated that mRNA accumulation of SlDHN2b was induced by salt stress,abscisic acid(ABA)and dehydration. Analysis of the promoter of PdEPF1 revealed the presence of stress-responsive elements by genome walking kit. Tissue-specific expression indicated that SlDHN2b was mainly expressed in leaves,weakly expressed in bud,flower,stem and the lowest in roots. Analysis of the promoter of SlDHN2b revealed the presence of stress-responsive elements.
NETL
NSTL
万方数据
维普
