Expression of Transcriptional Factors and Structural Genes of Anthocyanin Biosynthesis in Purple-heading Chinese Cabbage
To investigate the mechanism of anthocyanin production in our new purple-heading Chinese cabbage[Brassica campestris L. ssp. pekinensis(Lour)Olsson],the expression of structural genes and regulatory genes involved in anthocyanin biosynthesis was examined between the purple line (11S96)and its non-purple parent(09S17)by the real-time fluorescent quantitative RT-PCR,as well as their leaves cross-section were observed and the total anthocyanin content was measured. Leaf cross-section showed that the anthocyanin distribution is non-uniform in the purple heading leaf,specially enriched in the epidermis and its adjacent mesophyll cells. Comparison with the negligible amount of anthocyanin in the non-purple parent,the anthocyanin in purple line is up to 0.79 mg · g^-1 FW in purple inner heading leaves. Q-PCR analysis indicated that the EBGs(Early Biosynthesis Genes)CHS,CHI,F3H,F3′H,LBGs(Late Biosynthesis Genes)UFGT and GST,as well as the regulatory gene MYB0 were up-regulated in the inner leaves of purple heading;the other three LBGs,DFR,ANS,LDOX and the regulatory genes MYB2,MYB4,MYB12 and MYB111 were high up-regulated in all the leaves of the purple heading,which means that there is probably close relationship between these genes and the anthocyanin accumulation in this purple-heading Chinese cabbage,and MYB2 and MYB4 may play the dominant role in it.
Chinese cabbagepurple-headinganthocyanindistributiongene expression analysis
NETL
NSTL
万方数据
维普
