套袋对砀山酥梨果实石细胞发育及木质素代谢的影响
Bagging for the Development of Stone Cell and Metabolism of Lignin in Pyrus bretschneideri ‘Dangshan Suli'
王斌 1张楠 1闫冲冲 1金青 1林毅 1蔡永萍 1张金云2
作者信息
- 1. 安徽农业大学生命科学学院,合肥230036
- 2. 安徽省农业科学院园艺研究所,合肥230031
- 折叠
摘要
以砀山酥梨为材料,于花后15 d对果实套袋,观察不同发育时期果实石细胞团分布,测定石细胞、木质素及木质素中间代谢产物含量及木质素代谢相关酶活性,以期探讨套袋对梨石细胞发育和果实品质的影响.结果表明:套袋后,砀山酥梨果实石细胞含量下降31.6％,石细胞团数量减少28.0％,石细胞团直径减小45.8％.木质素含量下降13.9％,木质素含量与石细胞含量极显著正相关.梨果实发育的整个时期,套袋果实中PAL、C4H、4CL和CAD活性受到抑制,木质素中间代谢产物肉桂酸、香豆酸、咖啡酸和阿魏酸含量降低.套袋使木质素合成减少,石细胞发育受到阻碍.砀山酥梨果实木质素合成可能主要是通过香豆酸途径.
Abstract
To study the impact of bagging on pear fruit quality and the development of stone cell, the fruits of Pyrus bretschneideri 'Dangshan Suli' were bagged at the 15 days post anthesis and collected at different fruit stages to observe the distribution of sclereid and examine the content of stone cell> lignin and intermediate metabolite of lignin and the activities of relative enzymes. The results were as following: The bagged fruits decreased their stone cell content and lignin content by 31.6% and 13.9%, besides the quantity and diameter of stone cells decreased by 28.0% and 45.8%. The sclereid content and lignin content were extremely significant positive correlation. In the entire period of the development of pear fruit, the PAL, C4H, 4CL and CAD activities of bagged fruit were suppressed; Lignin intermediate metabolite of cinnamic acid, coumaric acid, caffeic acid, ferulic acid content decreased. The bagging reduced lignin synthesis and the development of sclereid has been hampered. The lignin synthesis of Dangshan Suli pear fruit may be mainly through coumaric acid pathway.
关键词
梨/套袋/石细胞/木质素代谢Key words
Pyrus bretschneideri/bagging/stone cell/lignin metabolism引用本文复制引用
基金项目
国家自然科学基金(31171944)
国家自然科学基金(30771483)
出版年
2013
国家科技期刊平台
NSTL
维普
万方数据