Analysis of Nuclear Genome Codon Preference in Cabbage
Forty-eight thousand coding DNA sequences of cabbage were used to analyze the relative frequency of synonymous codon (RFSC).Six high-frequency codons including TCT,CCT,AGA,GTT,GCT,and GAT were revealed by CodonW.When the frequency of codon usage of cabbage was compared to Escherichia coli,Arabidopsis thaliana,and Oryza sativa,we found that the codon preference was considerably different between cabbage and Escherichia coli,Oryza sativa,while the codon preference was identical between cabbage and Arabidopsis thaliana,Gossypium hirsutum.The codons of Bt crylC gene sequence have been reconstructed according to the results.The foreign gene containing the optimal codons of cabbage is helpful to researches involving cabbage transformation.