Forecasting Analysis of the Grape Flowering Pathway Based on the Grape EST Database
Homologous grape EST sequences were searched against the grape EST database using the corresponding flowering pathway gene sequences from Arabidopsis as the probes.The existence and expression of related genes involved in the flowering pathway in grape were further studied by half-qRT-PCR using eDNA,prepared from different grape organs at various developing stages,as PCR template,bashed on which,the grape flowering pathways were thoroughly forecasted.The study results also indicated that the expression levels of the grape flowering pathway genes showed some spatiotemporal differences.