首页|紫菜薹、紫色芜菁和紫色白菜花青苷分析

紫菜薹、紫色芜菁和紫色白菜花青苷分析

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在对紫菜薹(Brassica rapa L.ssp.chinensis var.purpurea Baile.)以及芜菁(Brassica rapa ssp.rapifera Metzg.)和白菜[Brassica rapaL.ssp.chinensis (L.) Makino]中的紫色品种类型叶片中花青苷分布特点研究的基础上,结合利用UFLC-UrV-Q-Trip-MS和UP,LC-Q-TOF-MS两种液相色谱质谱联用(LC-MS)技术,对叶片中花青苷代谢物谱进行分析鉴定.结果表明,花青苷在紫菜薹、紫色芜菁和紫色白菜叶片中积累的部位并不相同,主要分布于紫菜薹和紫色芜菁叶柄的表皮细胞,以及紫色白菜叶片的上表皮细胞中.3种蔬菜中共鉴定出23种花青苷,其中紫菜薹和紫色白菜含有的花青苷种类相同,为17种不同酰基化取代的矢车菊素-3-双/三葡萄糖苷-5-葡萄糖苷;紫色芜菁中检测出与紫菜薹和紫色白菜不同的6种花青苷,为不同酰基化的天竺葵素-3-双葡萄糖苷-5-葡萄糖苷.
The Anthocyanin Metabolic Profiling Analysis of Three Purple Brassica rapa Vegetables
The purple color of purple flowering Chinese cabbage (Brassica rapa L.ssp.chinensis var.purpurea Baile.) as well as purple types of turnip (Brassica rapa ssp.rapifera Metzg.) and pakchoi [Brassica rapa L.ssp.chinensis (L.) Makino] is due to anthocyanin accumulation.To investigate the distribution and metabolic profiling of anthocyanins in the leaves of these vegetables,tissue sections of leaves were observed,as well as the analytical method combining two LC-MS techniques,UFLC-UV-Q-Trap-MS and UPLC-Q-TOF-MS,were performed for the identification of anthocyanins.The anthocyanin pigments were mainly accumulated in the petiole epidermis of purple flowering Chinese cabbage and purple turnip,while in the upper epidermis of blades of purple pakchoi.In total,23 anthocyanins were detected and identified in the leaves of these vegetales.Seventeen different acylated cyanidin 3-di/triglucoside-5-glucosides were detected and identified in purple flowering Chinese cabbage and purple pakchoi.Six anthocyanins in purple turnip were identified as different acylated pelargonidin 3-diglucoside-5-glucosides.Based on these results the metabolic profiling of anthocyanins was extensively characterized in these three purple Brassica rapa vegetables.

purple flowering Chinese cabbageturnippakchoianthocyaninmetabolic profiling analysis

郭宁、郑姝宁、武剑、程锋、梁建丽、王晓武

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中国农业科学院蔬菜花卉研究所,北京100081

紫菜薹 芜菁 白菜 花青苷 代谢谱分析

国家‘863’计划项目国家自然科学基金

2012AA10010131301771,31201628

2014

园艺学报
中国园艺学会 中国农业科学院蔬菜花卉研究所

园艺学报

CSTPCDCSCD北大核心
影响因子:1.127
ISSN:0513-353X
年,卷(期):2014.41(8)
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