首页|耧斗菜HSF分类、表达分析及AyHSF1的亚细胞定位

耧斗菜HSF分类、表达分析及AyHSF1的亚细胞定位

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从蓝花耧斗菜(Aquilegia coerulea)基因组中鉴定出了14个热激转录因子(Heat shock transcription factor,HSF),对其进行了序列和进化树分析,发现该物种中有8个A类、5个B类和1个C类HSF.荧光定量PCR表明:14个预测的HSF基因在华北耧斗菜(A.yabeana)中均有表达,其中大部分成员表达响应热激处理.从华北耧斗菜cDNA中克隆了HSF1,构建了GFP表达载体,通过洋葱表皮细胞瞬时表达,发现GFP-AyHSF1融合蛋白存在转位现象.
Classification and Gene Expression Analysis of HSFs from Aquilegia coerulea and Sub-cellular Localization of AyHSF1
Fourteen HSFs (Heat shock transcription factors) were identified from Aquilegia coerulea genome sequence.Alignment and phylogenetic analyses displayed there were 8 HSFAs,5 HSFBs and 1 HSFC in A.coerulea.Real-time PCR revealed that transcripts of these HSFs were detected and most of them were induced significantly by heat shock in A.yabeana.Furthermore,HSF1 was cloned from A.yabeana cDNA.The protein fused with GFP at its N-terminus was investigated in onion epidermal cell and observed with a translocation phenomenon.

Aquilegia coeruleaA.yabeanaheat shock transcription factor (HSF)heat shockthermotolerance

张华丽、王涛、崔荣峰、董爱香、辛海波、义鸣放

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北京市园林科学研究院,绿化植物育种北京市重点实验室,北京100102

中国农业大学观赏园艺与园林系,北京100193

蓝花耧斗菜 华北耧斗菜 热激转录因子 热激 耐热性

农业部‘948’引进项目北京市公园管理中心项目

2011-G17ZX2014026

2015

10.16420/j.issn.0513-353x.2014-1150

园艺学报
中国园艺学会 中国农业科学院蔬菜花卉研究所

园艺学报

CSTPCDCSCD北大核心
影响因子：1.127
ISSN：0513-353X
年,卷(期)：2015.42(8)
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