Observation on Histological Structure of Strawberry Roots After Inoculating Fusarium oxysporum f.sp.fragariae Under 4-hydroxybenzoic Acid Stress
Two strawberry (Fragaria × ananassa Duch.) cultivars Toyonoka and Tutela as material, we studied the histological structure of strawberry roots, which infected by F.oxysporum f.sp.fragariae under the autotoxin 4-hydroxybenzoic acid stress by using paraffin slice.The results showed that the infection rate of hyphae of F.oxysporum f.sp.fragariae in root tissues of Toyonoka and Tutela under 4-hydroxybenzoic acid stress was faster than the treatment of only fungi-inoculated.The damages to the epidermis cells, cortex cells, stele cells and duct walls of Toyonoka and Tutela roots were more serious than the treatment of only fungi-inoculated, respectively.Moreover, the damage to the above-mentioned root cells of Toyonoka was more serious than that of Tutela under 4-hydroxybenzoic acid stress.The proportions of vessel infected by hyphae both in the roots of Toyonoka and Tutela were increased significantly under 4-hydroxybenzoic acid stress, with 22.5％ and 15.8％, respectively.The proportions of catheter with jelly was 26.3％ in Tutela roots, which was higher than in Toyonoka roots with 16.3％.Under 4-hydroxybenzoic acid stress, the tylosis produced in root vessels of Tutela earlier than in root vessels of Toyonoka.The proportions of vessel with tylosis in roots of Tutela was 1.6％, higher significantly than in Toyonoka with 1.0％ at both of them treated only by inoculating F.oxysporum, and higher significantly than in Tutela with 1.2％ treated by inoculating F.oxysporum under 4-hydroxybenzoic acid stress.The damage to the strawberry root tissue significantly increased after inoculating F.oxysporum under 4-hydroxybenzoic acid stress, and Toyonoka roots wilt disease was more serious.
NETL
NSTL
万方数据
维普
