首页|辣椒全基因组WRKY转录因子的分析

辣椒全基因组WRKY转录因子的分析

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基于已公布的辣椒全基因组数据,利用生物信息学方法对辣椒WRKY转录因子家族进行全面鉴定和系统命名,并在此基础上对基因分类、染色体定位、系统进化关系和结构域序列保守性进行了研究.结果表明:辣椒CaWRKY家族包含71个基因,根据WRKY结构域的数量及锌指结构的特征可将其分为Group Ⅰ、GroupⅡ和GroupⅢ等3大类,Group Ⅱ又可分为Ⅱ (a)、Ⅱ (b)、Ⅱ (c)、Ⅱ (d)和Ⅱ (e)等5个亚类.辣椒12条染色体上均有WRKY转录因子分布,其中第1号染色体上分布最多,共有10个,第4号染色体上分布最少,仅有2个.辣椒每类/亚类WRKY几乎含有相同的保守基序.辣椒WRKY编码的蛋白在132 ~ 869个氨基酸范围内,平均氨基酸数量为373个.
Genome-wide Analysis of the WRKY Transcription Factor Family in Pepper
In the present study, based on the recently released pepper whole-genome sequences, CaWRKY gene family identification, gene classification, chromosome location, sequence alignment and conserved structure domains of CaWRKY proteins were predicted and analyzed with bioinformatics methods.The results showed that 71 CaWRKYgenes were identified which were classified into three main groups (Ⅰ , Ⅱ and Ⅲ), with the second group further divided five subgroups [Ⅱ (a), Ⅱ (b), Ⅱ (c), Ⅱ (d) and Ⅱ (e)].A total of 70 CaWRKY genes were mapped to 12 chromosomes, whereas only CaWRKY70 was not mapped to any particular chromosome.Genome mapping analysis revealed that pepper WRKY genes were enriched on several chromosomes (1, 2, 3 and 7), especially on chromosome 1 which encompasses the largest number of 10 CaWRKY genes, while chromosome 4 only contained 2 CaWRKY genes.The pepper WRKYs from each group or subgroup were shown to share similar motif compositions, and CaWRKY proteins contained 132-869 amino acids and 373 in average.Our results will provide a platform for functional identification and molecular breeding tudy of WRKY genes in pepper.

pepperWRKYtranscription factorbioinformatic

刁卫平、王述彬、刘金兵、潘宝贵、郭广君、戈伟

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江苏省农业科学院蔬菜研究所,江苏省高效园艺作物遗传改良重点实验室,南京 210014

辣椒 WRKY 转录因子 生物信息学

国家高技术研究发展计划(‘863’计划)项目江苏省自然科学基金国家现代农业产业技术体系建设专项资金项目江苏省农业科技自主创新资金体系类项目

2012AA1001032014 BK1380CARS-25CX121004

2015

园艺学报
中国园艺学会 中国农业科学院蔬菜花卉研究所

园艺学报

CSTPCDCSCD北大核心
影响因子:1.127
ISSN:0513-353X
年,卷(期):2015.42(11)
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