Identification and Expression Analysis of MADS-box Genes Related to Female and Male Flowers Development in Chestnut
To screen key genes involved in the differentiation and development of flowers in chestnut (Castanea mollissima Blume),17 MADS-box genes were identified from the differentially expressed genes (DEGs) between female and male flowers of the mixed inflorescence by RNA-seq analysis.Gene structure analysis showed that two genes were Ⅰ-type MADS box genes as lack of semi-conserved K motif,while the other fifteen genes belonged to Ⅱ-type MADS-box group.And phylogenetic analysis demonstrated that among the 13 MADS-box genes with complete open reading frames (ORFs),there were two B-class genes,one C/D-class gene and three E-class genes based on the ABCDE model hypothesis.Furthermore,quantitative real time PCR was used to investigate the expression profiling of these genes in young leaves,male inflorescences at early stage,male and female flowers in the mixed inflorescence.And for one thing the results confirmed that these genes were DEGs (fold change ＞ 2),for another it indicated that some of these MADS-box genes showed very similar expression pattern.And it assumed that these MADS-box genes might play important roles in the differentiation and development of flowers in chestnut.
NETL
NSTL
万方数据
维普
