Analysis of Interactions Between AGL18 Family Members and Flowering Time Integrator Factor SOC1 in Brassica juncea
AGAMOUS-LIKE 18 (AGL18) is a key regulatory factor in flowering time control of Brassicajuncea.In order to clarify the regulatory mechanism of AGL18 protein interact with SOC1 in flowering pathways,AGL18-1 gene from reproductive shoots as well as AGL18-2 and AGL18-3 from vegetative shoots in ‘QJ’ germplasm of Brassicajuncea,were cloned respcetively.AGL18-1,AGL18-2 and A GL18-3 were members of AGL 18 family and respectively encoded 257,257 and 258 amino acids.Sequences analysis showed that they were more than 90％ similar to Brassica rapa and Brassica napus.Both Yeast two hybrid and BiFC showed that SOC1 protein could not interact with AGL18-1,AGL18-2 or AGL18-3.Yeast one hybrid and Dual-Glo(R) Luciferase assays indicated that SOC1 promoter could interact with AGL 18-1 but not AGL 18-2 and AGL 18-3.To screen the interaction regions of AGL 18-1/SOC1,the M-domain and IKC-domain truncations of AGL18-1 protein were separated and found that only the M-domain truncation could interact with SOC1 promoter.It suggested that M-domain was the key region that mediated the interaction between AGL18-1 and SOC1 promoter.The work provided valuable information for in-depth studies on the molecular mechanisms of AGL18 protein with SOC1 and the flowering time in Brassicajuncea.
NETL
NSTL
万方数据
维普
