Determination of Biological Characteristics and Preliminary Screening of Control Fungicides of Fusarium acuminatum Causing Fusarium Fruit Rot on Blueberry
The biological characteristics and fungicide control of Fusarium acuminatum causing Fusarium fruit rot were studied.The results of biological characteristics showed that the optimal media for mycelium growth and spore production were PSA and OA,respectively.The optimal temperature was 20 ℃ for mycelium growth,and 21 ℃ for spore production.Maltose and CMC-Na were optimal carbon sources for mycelium growth and spore production,respectively.Yeast powder was optimal nitrogen source for mycelium growth,and urea was suitable for spore production.The optimum pH for mycelium growth was 5 to 6,and the optimal pH for spore production was 6.The full darkness was suitable for mycelium growth.Under full light condition,the pathogen could produce more abundant spores.The mvcelial lethal temoerature was at 51 ℃ for 10 min.The spore lethal temperature was at 67 ℃ for 10 min.The results of orthogonal test showed that temperature was the most important influence factor on colony diameter.The sensitivity of F.acuminatum to 21 fungicides was detected by mycelium growth rate method.The results showed that the pathogen was more sensitive to Paenibacillus polymyxa,Bacillus cereus,fludioxonil,propiconazole · azoxystrobin and B.subtilis (EC50 < 0.5000 mg· L-1).The results of control effect experiment showed that fludioxonil and Paenibacillus polymyxa with lower disease incidence and lesion diameter had better control effects,and could effectively control Fusarium fruit rot on blueberry.