Cloning and Abiotic Stress Expression Analysis of CsMAPK3 Gene in Tea Plant
MAPK (mitogen-activated protein kinase) genes play a crucial role in the plant response to stress.In this study,the full-length of cDNA and genome sequence,and the promoter sequence of CsMAPK3 were isolated from the tea plant (Camellia sinensis) cultivar ‘Fudingdabai’.Moreover,the characteristic of bioinformatics and the expression patterns of CsMAPK3 under different stress treatments were investigated.The full-length cDNA of CsMAPK3 was 1 700 bp,with a 1 119 bp ORF,encoding 373 amino acids (Accession No.MF034662).It was predicted that CsMAPK3 was a hydrophilic protein,containing multiple phosphorylation sites.The homologous alignment and phylogenetic tree analysis showed that CsMAPK3 had a CD domain in the C-terminal region and conserved in TEY motif,which belongs to the group A of MAPKs.Subcellular localization prediction suggested that CsMAPK3 could be located in both the cytoplasm and the nucleus.The genome sequence of CsMAPK3 was 4 930 bp in length and constituted by five introns and six exons;thereinto,the first and the second intron were 1 608 bp and 1 318 bp in length,respectively,which were longer than other introns,whereas the length of the exons was ranged from 130 bp to 350 bp.Moreover,we cloned a promoter sequence of CsMAPK3 which was 1 125 bp in length,and contained several stress-responsive elements involved in drought,cold,high temperature and ABA-signaling.Expression analysis showed that ABA,cold and salt treatments could significant up-regulate the expression of CsMAPK3.Protein interaction network prediction showed that CsMAPK3 could interact with MYBR1 resulted in responsing to abiotic stress in ABA-dependent pathway.In conclusion,CsMAPK3 might be correlated to the abiotic stress-responsive in tea plant.
NETL
NSTL
万方数据
维普
