首页|百合抗病无性系抗枯萎病的蛋白质组学分析

百合抗病无性系抗枯萎病的蛋白质组学分析

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以百合抗病无性系和感病无性系为试验材料,开展了百合抗尖孢镰刀菌枯萎病的蛋白质组学研究,以期明确抗病无性系参与抗病反应的关键蛋白.利用蛋白质双向凝胶电泳技术,对被百合尖孢镰刀菌诱导0、24、48和72 h的抗病无性系和感病无性系进行差异蛋白分析.经质谱检测及数据库检索,鉴定了25个差异蛋白质点,其中10个鉴定为未知功能的蛋白,其余15个依据其相关功能分为4类,即防御反应相关蛋白、光合作用相关蛋白、糖类及能量代谢相关蛋白和热激蛋白.其中参与防御反应的病程相关蛋白(推定的抗病蛋白RPS2、推定的抗晚疫病同族蛋白R1C-3、过氧化物酶Q、抗坏血酸过氧化物酶和NBS-LRR类似蛋白)可能是百合抗病无性系介入抗病防御反应的关键蛋白.
Proteomics Analysis of Lily Blight Disease-resistant Clones
To elucidate the key proteins related to disease-resistance of lily induced by Fusarium oxysporum f.sp.lilii,protein two-dimensional gel electrophoresis technology was used to analyze profiles of the disease-resistant and susceptible clones after inoculation of Fusarium oxysporum f.sp.lilii for 0,24,48 and 72 h.Tweenty-five differentially expressed proteins were successfully identified,including 10 differentially expressed proteins identified as unknown function proteins.The rest of the 15 differentially expressed proteins can be categorized into 4 classes according to the function,namely the defense reaction,photosynthesis,carbohydrate and energy metabolism,and heat shock-related proteins.The disease related proteins participated in the defense reaction including presumption of disease-resistant protein RPS2,putative late blight resistant protein homolog R1 C-3,peroxidase Q,ascorbate peroxidase and NBS-LRR-like protein,which may play key roles in the resistance against lily blight.

lilyprotein extractiontwo-dimensional electrophoresisFusarium oxysporum f.sp.liliidifferential proteomics

张艺萍、瞿素萍、杨秀梅、马璐琳、许凤、王继华、何月秋

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云南农业大学植物保护学院,昆明650205

云南省农业科学院花卉研究所,国家观赏园艺工程研究中心,云南省花卉育种重点实验室,云南省花卉工程技术研究中心,昆明650205

百合 总蛋白提取 双向电泳 尖孢镰刀菌百合专化型 差异蛋白质组

国家自然科学基金云南省科技人才和平台计划项目云南省科技创新人才计划——科技领军人才项目国家高技术研究发展计划“863”项目

312604842017HB0832016HA0052011AA100208

2018

园艺学报
中国园艺学会 中国农业科学院蔬菜花卉研究所

园艺学报

CSTPCDCSCD北大核心
影响因子:1.127
ISSN:0513-353X
年,卷(期):2018.45(3)
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