Development of SSR Molecular Markers and Construction of Core Collection of Wild Diploid Strawberry
Core collection can reduce the number of resources and maximize the genetic diversity of the population.The 106 829 SSR loci were obtained by searching the genome of Fragaria vesca L.with MISA program,and 5 778 unique and common SSR sequences were obtained by comparing the genome of eight diploid strawberries such as F.vesca L.Among the 26 complex repeats,18 SSR loci were found in F.vesca L,F chinensis Lozinsk,F.mandshurica Staudt,F.nilgerrensis Schlecht.,F.nubicola Lindl.,F.emeiensis Jia J.Lei,F.pentaphylla Lozinsk and F.viridis Duch.Among them,the single,two,three,four,five,six nucleotide types and complex repeat types were 3 461,1 223,999,57,7,5,26,respectively.Among 26 complex repeat types,18 SSR loci have high coverage in 176 diploid strawberry re-sequencing data,which are used for construction of core collection.The results showed that 444 alleles were obtained from 176 accessions of wild diploid strawberry with 18 pairs of conservative sequence,the average number of effective alleles was 7.899,the Shannon's information index was 0.836-3.362,the observed heterozygosity was 0.006-0.477,the expected heterozygosity was 0.324-0.953,the PIC value was 0.315-0.951,and the average value was 0.756.The core collection of wild diploid strawberry was constructed with 18 pairs of SSR primers selected,and the core collection was evaluated by t-test.The fourty-three accessions were selected,which preserved more than 80％of genetic diversity and were highly representative.They were identified as the core collection of wild diploid strawberry,and at the same time,the molecular ID of two-dimensional code of core collection was constructed.The polymorphic SSR loci developed in this study can provide research tools for identification and molecular marker breeding of wild strawberry in the future.
NETL
NSTL
万方数据
维普
