Expression of S-RNase in vitro and Identification of Its Interacting Protein FviFPA from Fragaria viridis
The self-incompatible style determinants Sa-RNase,Sb-RNase and their interacting protein FviFPA were studied based on the selfing progenies of Fragaria viridis 10-42 line.The results showed that the recombinant S-RNase protein obtained by prokaryotic expression technology could specifically inhibit self-pollen growth,both the pollen germination rate and pollen tube length decreased with the increase of the recombinant protein concentration.FviFPA protein that interacts with Sa-RNase was screened from Fragaria viridis style cDNA library in the early stage.In this study,the full-length of FviFPA was cloned and the bioinformatics was analyzed,the authenticity of the interaction between FviFPA and S-RNase was verified by yeast two-hybrid and bimolecular fluorescence complementation assay,and there was no haplotype specificity in the interaction.
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NSTL
万方数据
维普
