染色质免疫共沉淀测序技术及其在园艺植物中的应用研究进展
Advances in Chromatin Immunoprecipitation Sequencing and Its Application in Horticultural Plants
翟挺楷 1马湘玮 1陈燕 1张雪莹 1李卓蕴 1徐卢振 1傅卓然 1赖钟雄 1林玉玲1
作者信息
- 1. 福建农林大学园艺植物生物工程研究所,福州 350002
- 折叠
摘要
近年来,基于染色质免疫共沉淀技术(ChIP)与第二代测序技术结合的染色质免疫共沉淀测序技术(ChIP-seq)己被广泛用于研究DNA与蛋白质的相互作用,对解析园艺植物基因的表达与调控、靶基因筛选与定位、调控网络构建、染色质开放程度确认等方面具有较大的优势.本文综述了 ChIP-seq技术的发展简史及其在园艺植物转录因子和组蛋白修饰中所取得的进展,以期为园艺植物DNA—蛋白互作研究提供参考.
Abstract
In recent years,chromatin immunoprecipitation sequencing technology(ChIP-seq)based on the combination of chromatin immunoprecipitation technology(ChIP)and second-generation sequencing technology has been widely used to study the interaction between DNA and proteins.It has great advantages in analyzing the expression and regulation of horticultural plant genes,screening and mapping of target genes,construction of regulatory networks,and confirmation of chromatin openness.This paper reviews the development history of ChIP-seq technology and its progress in the modification of transcription factors and histones in horticultural plants,with the aim of providing theoretical reference for the study of DNA-protein interactions in horticultural plants.
关键词
DNA与蛋白质互作/染色质免疫共沉淀测序/转录因子/组蛋白修饰Key words
DNA and protein interaction/chromatin immunoprecipitation sequencing/transcription factor/histone modification引用本文复制引用
基金项目
国家自然科学基金项目(31672127)
福建省自然科学基金项目(2020J01543)
福建省高原学科建设经费项目(102/71201801101)
福建农林大学创新基金项目(KFb22022XA)
福建农林大学创新基金项目(CXZX2019033S)
出版年
2024
NETL
NSTL
维普
万方数据