Mapping of Gene Regulating Citric Acid Content in Tomato Fruit
The content of citric acid in tomato fruit is one of the key factors affecting the flavor.Due to the citric acid content is greatly affected by the environment few genes related with citric acid content have been cloned.In this study,six QTLs,which control the content of citric acid,were identified using a recombinant inbred lines generated by crossing modern cultivated tomato'Moneymaker'and S.pimpinellifolium'PI365967'.Among them,q-CA6.1,a major QTL located at the terminal of chromosome 6,contributed 19.28%variation of citric acid content.To map qCA6.1 gene a BC2F2 mapping population was obtained by crossing two RILs with significant difference in citric acid content and with similar genotype and the difference only around the qCA6.1 region.The qCA6.1 gene were narrowed into 342 kb region including 47 gene.The development of linked molecular markers provides technical support for improving citric acid content in tomato breeding.