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调控番茄果实柠檬酸含量的基因定位

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为了挖掘调控番茄果实柠檬酸含量的关键基因,以栽培番茄(Solanum lycopersicum'Moneymaker')和野生醋栗番茄(Solanum pimpinellifolium'PI365967')为亲本构建的重组自交系群体为材料,利用混池重测序技术挖掘到6个控制柠檬酸含量的QTL。其中位于6号染色体末端的主效QTL qCA6。1表型贡献率高达19。28%。为对该位点进行定位,从重组自交系群体中挑选出2份柠檬酸含量差异较大,且只在主效位点存在基因型差异的株系作为亲本,构建BC2F2定位群体,通过交换单株后代鉴定,将定位区间缩小到342 kb区间内,并开发了连锁分子标记,为后续克隆基因奠定了基础,也为改良番茄果实柠檬酸含量提供了重要选择标记。
Mapping of Gene Regulating Citric Acid Content in Tomato Fruit
The content of citric acid in tomato fruit is one of the key factors affecting the flavor.Due to the citric acid content is greatly affected by the environment few genes related with citric acid content have been cloned.In this study,six QTLs,which control the content of citric acid,were identified using a recombinant inbred lines generated by crossing modern cultivated tomato'Moneymaker'and S.pimpinellifolium'PI365967'.Among them,q-CA6.1,a major QTL located at the terminal of chromosome 6,contributed 19.28%variation of citric acid content.To map qCA6.1 gene a BC2F2 mapping population was obtained by crossing two RILs with significant difference in citric acid content and with similar genotype and the difference only around the qCA6.1 region.The qCA6.1 gene were narrowed into 342 kb region including 47 gene.The development of linked molecular markers provides technical support for improving citric acid content in tomato breeding.

tomatoflavorcitric acidrecombinant inbred linesQTL

田茂森、周震、王海敬、崔霞、孙帅

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青岛农业大学园艺学院,山东青岛 266109

中国农业科学院蔬菜花卉研究所,蔬菜生物育种全国重点实验室,北京 100081

番茄 风味 柠檬酸 重组自交系 QTL

国家自然科学基金重点国际(地区)合作研究项目

32120103010

2024

园艺学报
中国园艺学会 中国农业科学院蔬菜花卉研究所

园艺学报

CSTPCD北大核心
影响因子:1.127
ISSN:0513-353X
年,卷(期):2024.51(1)
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