Cloning and Expression Analysis of Mg-Chelatase H Subunit Gene CsChlH in Tea Plant(Camellia sinensis)
In this study,the complete open reading frame sequence of CsChlH gene encoded 1 382 amino acid residues was cloned in tea plant(NCBI accession number:ON256194).Phylogenetic tree analysis showed that CsChlH was closely related to AcChlH of kiwifruit.Promoter cloning and sequence analysis showed that CsChlH gene may respond to multiple signals such as light,drought,heat shock,low temperature,ABA,ethylene,and may regulate the metabolism of chlorophylls,flavonoids,sugars,as well as the development of chloroplast,mesophyll,guard cell and other organs.CsChlH protein was localized in the chloroplast.Tissue-specific expression analysis found that CsChlH gene was highly expressed in the chlorophyll-rich source tissues but lowly expressed in the chlorophyll-deficient sink tissues.The expression of CsChlH gene was down-regulated in response to long-term low light conditions,cold and drought stresses,while up-regulated by short-term ABA,ethylene and long-term heat stress.The results revealed that CsChlH gene played a vital role in the regulation of chlorophyll metabolism and stress resistance of tea plant.
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万方数据
维普
