Effect of Boron Deficiency on the Root Growth and Mitochondrial Function of Trifoliate Orange Seedlings
The aim of this study was to clarify the mechanism of boron(B)deficiency on the root injury of trifoliate orange[Poncirus trifoliata(L.)Raf.]seedlings.Trifoliate orange seedlings were incubated in nutrient solution containing different levels of B(0 and 10 μmol·L-1)of which 0 μmol·L-1 was considered as deficient B and 10 μmol·L-1 was considered as control.Root growth parameters,mitochondrial reactive oxygen species metabolism,antioxidant enzyme activities,and mitochondrial function and ultrastructure were measured in this study.The results showed that B deficiency treatment inhibited root growth and activity,and the total root length,root surface area,root volume,average root diameter and biomass decreased significantly.Boron deficiency treatment increased the concentrations of hydrogen peroxide(H2O2)and malondialdehyde(MDA)but significantly decreased superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activities in root mitochondria.Boron deficiency treatment also reduced the change of mitochondrial membrane absorbance,indicating that the openness of mitochondrial permeability transition pore was increased.Boron deficiency treatment decreased mitochondrial membrane fluidity,membrane potential and cytochrome c/a,and signifiicantly reduced ATP synthesis due to impaired mitochondrial function.The transmission electron microscopy results showed that the root system's mitochondrial structure was damaged due to B deficiency treatment,causing the reduced mitochondrial crest and the occurrence of cavitation.In summary,B deficiency treatment significantly inhibits the growth and development of trifoliate orange seedlings,and its mechanism may be related to the damage of mitochondrial structure and function.
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万方数据
维普
