缺硼胁迫对枳幼苗根系生长及线粒体功能的影响
Effect of Boron Deficiency on the Root Growth and Mitochondrial Function of Trifoliate Orange Seedlings
金天 1徐月美 1邝冠翎 1刘桂东2
作者信息
- 1. 赣南师范大学生命科学学院,江西赣州 341000
- 2. 赣南师范大学生命科学学院,江西赣州 341000;国家脐橙工程技术研究中心,江西赣州 341000
- 折叠
摘要
为明确缺硼胁迫对枳[Poncirus trifoliata(L.)Raf.]幼苗根系损伤的机制,采用营养液培养法研究缺硼对枳幼苗根系生长发育、线粒体活性氧代谢、抗氧化酶活性变化、线粒体特性和线粒体超微结构的影响.结果表明:缺硼处理抑制了枳幼苗根系生长,根系总根长、总表面积、总体积、平均直径及根系生物量均明显降低,根系活力也显著下降.缺硼处理降低了根系线粒体超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性,增加了过氧化氢(H2O2)和丙二醛(MDA)含量.缺硼处理还使线粒体膜吸光度变化值降低,说明增大了线粒体膜透性转换孔开放程度.缺硼处理降低了线粒体膜流动性、线粒体膜电位和细胞色素c/a值,线粒体功能受损导致ATP合成明显减少.透射电镜观察结果显示缺硼处理的根系线粒体结构被破坏,线粒体内嵴减少,出现空泡化现象.综上,缺硼会显著抑制枳幼苗的生长发育,其作用机制可能与线粒体结构与功能受损有关.
Abstract
The aim of this study was to clarify the mechanism of boron(B)deficiency on the root injury of trifoliate orange[Poncirus trifoliata(L.)Raf.]seedlings.Trifoliate orange seedlings were incubated in nutrient solution containing different levels of B(0 and 10 μmol·L-1)of which 0 μmol·L-1 was considered as deficient B and 10 μmol·L-1 was considered as control.Root growth parameters,mitochondrial reactive oxygen species metabolism,antioxidant enzyme activities,and mitochondrial function and ultrastructure were measured in this study.The results showed that B deficiency treatment inhibited root growth and activity,and the total root length,root surface area,root volume,average root diameter and biomass decreased significantly.Boron deficiency treatment increased the concentrations of hydrogen peroxide(H2O2)and malondialdehyde(MDA)but significantly decreased superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activities in root mitochondria.Boron deficiency treatment also reduced the change of mitochondrial membrane absorbance,indicating that the openness of mitochondrial permeability transition pore was increased.Boron deficiency treatment decreased mitochondrial membrane fluidity,membrane potential and cytochrome c/a,and signifiicantly reduced ATP synthesis due to impaired mitochondrial function.The transmission electron microscopy results showed that the root system's mitochondrial structure was damaged due to B deficiency treatment,causing the reduced mitochondrial crest and the occurrence of cavitation.In summary,B deficiency treatment significantly inhibits the growth and development of trifoliate orange seedlings,and its mechanism may be related to the damage of mitochondrial structure and function.
关键词
柑橘/枳/缺硼胁迫/根系生长/线粒体Key words
citrus/trifoliate orange/boron deficiency/root growth/mitochondria引用本文复制引用
基金项目
国家自然科学基金项目(32160746)
江西省自然科学基金重点项目(20212ACB205008)
大学生创新训练项目(CX220064)
出版年
2024
NETL
NSTL
维普
万方数据