Metabolomic Analysis of Garlic Seedling Under Drought Stress
Using high performance liquid chromatography tandem mass spectrometry extensively targeted metabolomics,garlic leaves metabolic components were detected and analyzed by principal component analysis,orthogonal partial least squares discriminant analysis,cluster analysis,enrichment analysis and correlation analysis.As a result,a total of 743 metabolites in 23 classes were identified,313 up-regulated and 430 down-regulated metabolites,with 134 differential metabolites,accounting for 18.03％of the overall metabolites.The highest number of up-and down-regulated differential metabolites were flavone,phenylpropanoids,flavonol,flavonoid,nucleotide and derivatives,amino acid and derivatives,carbohydrates,organic acid and derivatives,which were mainly enriched in the flavone and flavonol biosynthesis.The number of different metabolites of flavonoids in the leaves of garlic sprout after drought stress was higher and up-regulated.The metabolic pathways of garlic flavonoids were analyzed,and caffeate acid and tricin O-malonylhexoside were the most up-regulated metabolites.It was speculated that the increase of flavone and phenylpropanoids under drought stress significantly improved the drought resistance of garlic.
NETL
NSTL
万方数据
维普
