Identification and Mechanism Characterization of Antagonistic Actinomyces SD-64 Against Pepper Southern Blight
In this study,a strain of Streptomyces SD-64 was screened from the rhizosphere soil of peppers by plate confrontation method,which has strong antagonistic effects on Sclerotium rolfsii.It was identified by morphological observation and molecular biology method,and its control effect on pepper southern blight and its mechanism of action were evaluated.The crude extract from the fermentation broth of strain SD-64 was extracted.The plate bioassay showed that strain SD-64 the inhibition rate on S.rolfsii 99.27％.Based on morphology and 16S rDNA identification,SD-64 was identified as Streptomyces griseoaurantiacus.SD-64 could produce melanin and cellulase.Its fermentation products could affect the formation and germination of sclerotium,affect hyphal morphology,destroy the integrity of cell membrane,and increase the accumulation of reactive oxygen species(ROS)in hyphae.The test of control effect of in vitro and pot experiment showed that the fermentation solution of SD-64 could significantly reduce the incidence of pepper southern blight.The EC50 of the crude extract from SD-64 fermentation broth against S.rolfsii was 78.281 µg·mL-1.In conclusion,the strain SD-64 has good control effect on pepper southern blight and has the potential for development and application.
pepperSclerotium rolfsiibiological controlactinomyces16SrDNAmode of action
NETL
NSTL
万方数据
维普
