Analysis and Identification of Endogenous Viral Elements in Solanaceous Crop Genome
In this study,an analysis of endogenous virus elements(EVEs)were conducted from 35 open genome data sequences of six Solanaceae species,resulting in the identification of 17 suspected plant EVEs primarily from Caulmoviridae.The analysis revealed that TVCV sequences were present as large fragments in nearly all sampled Solanaceae genomes,with a content ranging from 0.01％to 0.32％,an average length of 500 bp to 2 300 bp and a total number of sequences ranging from 200 to 5 000.Additionally,the distribution and composition of endogenous TVCV sequences were investigated in the potato DM1-3 genome.The results found they were dispersed across 12 chromosomes with an average of 57 sequences on each chromosome.These endogenous sequences were compared with the TVCV genome,and although they did not cover the complete genome sequence of TVCV,they covered the complete coding region.Furthermore,the presence and distribution of TVCV were comfirmed in potato materials by RT-PCR followed by in situ hybridization.
NETL
NSTL
万方数据
维普
