建兰花叶病毒的遗传变异及其寄主适应性
Genetic Variability and Host Adaption of Cymbidium Mosaic Virus
关夏玉 1陈细红 2郭菁 2吕浩阳 3梁晨媛 3高芳銮3
作者信息
- 1. 福建农林大学生物农药与化学生物学教育部重点实验室,福州 350002;福建农林大学园艺学院,福州 350002
- 2. 福建省检验检疫技术研究重点实验室,福州海关技术中心,福州 350001
- 3. 福建农林大学生物农药与化学生物学教育部重点实验室,福州 350002
- 折叠
摘要
建兰花叶病毒(Cymbidium mosaic virus,CymMV)是危害兰花并造成重要经济损失的病毒之一.为揭示CymMV的遗传变异及其适应性进化特征,新测定了 16个CymMV石斛兰(Dendrobium nobile)分离物的外壳蛋白基因(coatprotein,CP)序列,并结合己报道的52个CymMV中国分离物CP基因序列进行遗传变异及进化分析.遗传多样性分析结果显示,68个CymMV分离物CP基因的核苷酸多样性(Pi)和单倍型多样性(Hd)平均值分别为0.039和0.992,表明该病毒具有较高的遗传多样性.分子变异分析(analysis of molecular variance,AMOVA)显示,CymMV变异源主要来自于病毒个体,约占总变异的71.72％.群体遗传结构分析显示,该病毒可以分为两个大簇(Cluster),CymMV建兰(Cymbidium ensifolium)分离物独立为一簇(Cluster 1),其他寄主分离物聚为另一大簇(Cluster 2),Cluster 2内相同寄主来源的病毒分离物倾向于相聚成簇.进一步的系统发育与性状关联分析结果显示CymMV与寄主来源存在显著的关联性.上述结果表明,CymMV的遗传变异具有寄主特异性,其适应性进化主要受寄主因素所驱动.
Abstract
Cymbidium mosaic virus(CymMV)is a highly destructive plant pathogen that inflicts significant losses on orchid production.This study investigates the genetic variability and adaptive evolution of CymMV.The coat protein(CP)gene of 16 CymMV isolates from Dendrobium nobile was sequenced and analyzed alongside published CP sequences of 52 CymMV isolates from various host plants in China.Analysis reveals a substantial genetic diversity within the CP gene,with an overall nucleotide diversity of 0.039 and a haplotype diversity of 0.992.AMOVA tests demonstrate that the molecular variation among individuals accounts for 71.72％of the total variation of CymMV.Population structure analysis classifies CymMV into two distinct clusters.Cluster 1 exclusively consists of CymMV isolates from Cymbidium ensifolium,while Cluster 2 encompasses CymMV isolates from multiple host species,yet isolates from the same host species tend to form subclusters.Furthermore,a robust association between the phylogeny and host species of CymMV is revealed through phylogeny-trait association analysis.Overall,the findings of this study indicate that the genetic variation of CymMV is specific to its host species,suggesting that its evolution is primarily driven by selection pressures imposed by its hosts.
关键词
建兰花叶病毒/外壳蛋白基因/遗传多样性/主成分判别分析/适应性进化Key words
Cymbidium mosaic virus/coat protein gene/genetic diversity/discriminant analysis of principal components/adaptive evolution引用本文复制引用
基金项目
福建省自然科学基金面上项目(2019J01653)
福建农林大学园艺学院青年学术骨干培养基金项目(102/722022011)
出版年
2024
NETL
NSTL
维普
万方数据