The F2 genetic segregating population was constructed via using high ascorbic acid(AsA)tomato accession TS-226 and low ascorbic acid tomato accession TS-228 as parents.The high-ascorbic acid and low-ascorbic acid pools were constructed respectively.Through bulked segregant analysis sequencing analysis(BSA-seq),the main QTL related to ascorbic acid was found,and the candidate gene related to ascorbic acid was located in the 58.00-60.15 Mb region of tomato chromosome 8.Furthermore,the ΔSNP-index data were analyzed to determine the main candidate gene SlPPO(Polyphenol oxidase)that controls the ascorbic acid content of tomato fruit.Using TS-228 as the background plant,transgenic lines with SlPPO overexpression and silencing were obtained by Agrobacterium-mediated transformation.Compared with the wild type,the total ascorbic acid content in the red ripe fruits of the overexpression lines OE-3 and OE-18 decreased by 18.89％and 26.56％,respectively.The total ascorbic acid content in the red ripe fruits of the silenced lines Ri-9 and Ri-16 increased by 37.53％and 63.93％relative to wild type,respectively.Taken together,SlPPO is the key gene to control the ascorbic acid content of tomato red ripe fruit,and plays a negative role in regulating fruit ascorbic acid content.
维普
万方数据