测定了301份番茄(Solanum lycopersicum L.)种质的叶片在离体24 h后的相对失水率,以鉴定其抗旱能力,并进行了全基因组关联分析(genome-wide association study,GWAS).共检测到3个与番茄抗旱性显著关联的SNP位点,分别为S02.1158758、S02.1160893和S05.1426646,单个位点可以解释4.08％～18.97％的表型变异.挖掘到3个与番茄抗旱性显著关联的主效SNP位点和7个候选基因,有助于解析番茄抗旱性的遗传基础及其调控机制,为番茄抗逆遗传改良奠定了基础.
Abstract
To conduct genome-wide association studies(GWAS)of drought tolerance,the relative water loss of detached leaves was analyzed after 24 h dehydration in 301 tomato(Solanum lycopersicum L.)accessions.A total number of three SNP sites(S02.1158758,S02.1160893,and S05.1426646)significantly associated with the drought tolerance of tomato accessions were identified by GWAS.Each single locus could explain 4.08％-18.97％of the phenotypic variation.Taken together,three SNP sites and seven candidate genes significantly associated with drought tolerance in tomato were identified in this study,which will help unravel the genetic and regulatory mechanisms of tomato drought tolerance,as well as establish a foundation for the genetic improvement of tomato stress tolerance.
关键词
番茄/抗旱性/全基因组关联分析/候选基因
Key words
tomato/drought tolerance/genome-wide association studies/candidate gene
维普
万方数据