首页|桃镁离子转运蛋白MGT基因家族鉴定与表达分析

桃镁离子转运蛋白MGT基因家族鉴定与表达分析

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为研究桃树镁离子转运蛋白(Magnesium Transporters，MGT)家族基因在镁离子运输中所起的作用，全基因组鉴定、分析了桃树MGT家族成员(PpMGT)，并研究外源施镁对PpMGT基因表达的影响。通过同源比对、保守位点分析鉴定获得了 8个PpMGT，在Chr1、Chr3、Chr6、Chr8染色体上不均匀分布。系统发育研究表明植物MGT家族可分为5个分支，各分支成员数存在差异。基因和蛋白结构分析发现PpMGT含有4～13个外显子，蛋白中存在10个保守基序，启动子上游分布有不同数量的胁迫响应、转录调控、节律调控、激素响应和发育调控元件。PpMGT在桃树花、果、叶、根器官均有表达且具有组织表达特异性。桃树外源喷施MgCl224h后，检测到PpMGT转录表达变化。喷施镁使叶片、果皮出现不同的差异表达基因类群，均涉及光合途径。研究发现PpMGT4、PpMGT6、PpMGT8与喷镁后光系统Ⅱ捕光叶绿素a/b蛋白复合体基因表达变化正相关。异源表达 PpMGT4、PpMGT6、PpMGT8可补偿Mg2+转运缺陷突变株MM281(鼠伤寒沙门氏菌突变株)生长缺陷，表明PpMGT4、PpMGT6、PpMGT8具有镁离子转运功能。根据序列特征、表达模式及外源镁喷施结果，推测PpMGT可能具有功能分化，通过协同作用调节桃树镁转运，PpMGT4、PpMGT6、PpMGT8的表达变化可能与外源镁摄入和利用相关。
Identification and Expressional Analysis of MGT Gene Family in Prunus persica
To investigate the roles of magnesium transporters(MGTs)in magnesium transportation,the genome-wide identification of peach(Prunus persica)MGTs(PpMGTs)was performed and their effects in exogenous magnesium application on peach tree were studied.A total of eight PpMGTs,which were unevenly distributed in four chromosomes(Chr1,Chr3,Chr6 and Chr8),were identified based on protein homology searches and conserved domain analyses.System evolution analysis results suggested that plant MGTs could be grouped into five different clades which show quantitative differences in species-specific MGTs.PpMGTs had 4-13 exons,ten conserved motifs in protein sequence,and diverse cis-elements of stress response,transcriptional regulation,circadian rhythm,phytohormone response and developmental growth in their upstream promoters.All PpMGTs were transcribed in flowers,fruits,leaves and roots with their unique tissue specific expression pattern.The distinct transcriptional changes of PpMGTs could be observed after exogenous magnesium spraying.Differentially expressed genes found before and after exogenous magnesium application were discrepant in peach leaves and peels,but some of them were commonly involved in the photosynthesis pathway.The transcriptional profile and correlation analysis results suggested that expressional changes of light-harvesting chlorophyll a/b protein complex genes were positively corelated with transcriptional levels of PpMGT4,PpMGT6 and PpMGT8.Heterologous expression of PpMGT4,PpMGT6 and PpMGT8 were able to rescue growth defects of magnesium transport mutant MM281(Salmonella typhimurium mutant),suggesting these three proteins were serving important magnesium transport functions.Thus,the results from sequence characteristics,expressional profiles and exogenous magnesium application showed that PpMGTs had potential functional diversity and synergistic effects in magnesium transport and utilization.The expressional changes of PpMGT4,PpMGT6 and PpMGT8 may associate with exogenous magnesium intake and magnesium utilization.

PrunuspersicaMgMGTgene expressionfunctional diversity

周平、颜少宾、郭瑞、金光

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福建省农业科学院果树研究所,福建省落叶果树工程技术研究中心,福州 350013

桃 镁 镁离子转运蛋白 基因表达 功能分化

福建省省属公益类科研院所基本科研业务费专项福建省省属公益类科研院所基本科研业务费专项福建省省属公益类科研院所基本科研业务费专项福建省省属公益类科研院所基本科研业务费专项福建省省属公益类科研院所基本科研业务费专项福建省省属公益类科研院所基本科研业务费专项现代农业产业技术体系建设专项福建省农科院科研项目福建省农科院科研项目福建省团队科技特派员专项

2022R10280092021R10280082021R102800112020R10280032018R1013-132017R1013-1CARS-30-Z-07YDXM2023001CXTD2021009-2

2024

10.16420/j.issn.0513-353x.2023-0056

园艺学报
中国园艺学会 中国农业科学院蔬菜花卉研究所

园艺学报

CSTPCD北大核心
影响因子：1.127
ISSN：0513-353X
年,卷(期)：2024.51(3)
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