嗜线虫镰刀菌NQ8G Ⅱ 4对枸杞根腐病的防效及其机制研究
Analysis of the Control Effect of Biocontrol Fungus Strain Fusarium nematophilum NQ8G Ⅱ 4 on Chinese Wolfberry Root Rot and Its Resistance Induction Mechanism
贾淑鑫 1李金 1闫思远 1郭苗苗 1王若彤 1顾沛雯1
作者信息
摘要
为明确内生真菌嗜线虫镰刀菌(Fusarium nematophiluim)NQ8G Ⅱ 4菌株对枸杞根腐病的防效及作用机制,利用平板对峙法测定其对枸杞根腐病原菌(Fusarium oxysporum f.sp.lycium barbarum)菌丝生长的抑制作用,通过盆栽试验测定对枸杞根腐病的防治效果,并采用防御酶活性测定和组织化学染色分析其作用机制.结果表明,预先3d接种NQ8G Ⅱ 4菌株能显著抑制尖孢镰刀菌菌丝的生长,抑制率高达70.07％.拌土法接种NQ8G Ⅱ 4菌株可显著降低温室中枸杞根腐病的发生,防效为76.38％,与化学药剂多菌灵防效相当.接种NQ8G Ⅱ 4菌株显著促进枸杞生长,株高、侧枝数、叶宽、茎粗和根长分别比对照增加了 37.61％、90.56％、52.50％、32.35％和44.69％.在病原菌胁迫下,接种NQ8G Ⅱ 4后21 d与不接种相比POD、SOD、PAL和CAT活性分别增加33.92％、65.64％、57.96％和41.59％,MDA含量减少44.23％.说明接种NQ8G Ⅱ 4菌株可以促进枸杞幼苗生长,激发植株体内防御酶活性,缓解病原菌胁迫导致的膜脂过氧化作用,进而提高对根腐病的抗性,该菌株具有良好的生防潜力和应用前景.
Abstract
To clarify the efficacy and mechanism of action of Fusarium nematophilum NQ8G Ⅱ 4 on Chinese wolfberry root rot,the inhibitory effect of F.nematophilum NQ8G Ⅱ 4 on the mycelial growth of F oxysporum f.sp.lycium barbarum was determined by the plate standoff method,and its effect on the control of chinese wolfberry root rot was determined by pot test,and the mechanism of action was analyzed by histochemical staining,the mechanism of action was analyzed by defense enzyme activity assay and histochemical staining.The results showed that pre-inoculation of strain NQ8G Ⅱ 4 for 3 d significantly inhibited the growth of F.oxysporum mycelium with an inhibition rate of 70.07％.The inoculation of NQ8G Ⅱ 4 strain with soil mixing method can significantly reduce the occurrence of Chinese wolfberry root rot in greenhouse,with 76.38％effectiveness,which is comparable to that of chemical carbendazim.Compared with the blank control,the agronomic traits of L.barbarum such as plant height,number of side branches,leaf width,stem diameter and root length increased by 37.61％,90.56％,52.50％,32.35％and 44.69％respectively after inoculation with NQ8G Ⅱ 4 strain,which could significantly promote the growth of L.barbarum.Under F oxysporum stress,leaf POD,SOD,PAL and CAT increased by 33.92％,65.64％,57.96％and 41.59％,respectively,while MDA content decreased significantly by 44.23％21 days after inoculation with NQ8G Ⅱ 4 compared to the control without NQ8G Ⅱ 4.Inoculation of strain NQ8G Ⅱ 4 promoted the growth of L.barbarum seedlings and stimulated the activity of defense enzymes in L.barbarum,and it stimulates the defense enzyme activity in L.barbarum and alleviates the membrane lipid peroxidation stress caused by F.oxysporum,thus improving the resistance of Chinese wolfberry root rot,which has good potential and application.
关键词
枸杞/根腐病/内生真菌/嗜线虫镰刀菌/生物防治Key words
Chinese wolfberry/root rot/endophytic fungi/Fusarium nematophilum/biocontrol引用本文复制引用
出版年
2024
NETL
NSTL
万方数据