Previous studies have shown that there are four SNP loci on the sequence of the russet peel associated SLAF tag(Marker286117),with a total of four genotypes(a,b,c,d)in Pyrus.In this study,PCR plus mixing pool re-sequencing was used to identify the polymorphisms of the SLAF tag in 64 pear cultivars.The results showed that a total of 7.1 Gb of raw data was obtained by re-sequencing,and the data were processed by two programs SNP-rseq and Calculate.The average number of reads obtained from 64 pear accessions was 117 642,which met the analysis needs.Two new genotypes(e and f)were found on this SLAF locus.Among six genotypes,genotype c was the original type,and genotypes b,d and e were produced in cultivated pears of P.pyrifolia and P.bretschneideri.Genotypes a and f appeared in samples of Western pear.When genotype and phenotype statistics showed that only genotype c existed,the peel of the pear fruit was russet,and those without genotype c were green.This rule applies not only to Eastern pears,but also to some Western pears.In this study,mixed-pool was used to reduce the cost of sequencing.This method could be applied to simplify the subsequent validation of re-sequencing and marking-assisted breeding.
NETL
NSTL
万方数据
