Screening and Evaluation of Reference Genes for Real-time Quantitative PCR in Wax Gourd
Three statistical software including GeNorm,NormFinder,and BestKeeper were applied to analyze the expression stability of nine internal reference genes(18SrRNA,ACT,CYP,EF-1α,F-box,GAPDH,TUA,TUB,and UBQ)in wax gourd.The plant samples of plants were taken in different tissues(root,stem,leaf,male flower,female flower,ovary),fruits at different developmental stages including(0 d,10 d,and 20 d after pollination)of wax gourd,and leaves under biotic stress(Phytophthora melonis infection),and abiotic stress(salt,high temperature,and low temperature treatment).The obtained results showed that the expression stability of EF-1α was the best in different tissues.The expression stability of F-box was the best in the fruits at different developmental stages and the leaves under P.melonis infection.By comparison,18SrRNA showed strong stability in the leaves under salt stress,while the expression stability of ACT was the best in the leaves under high temperature stress and TUA had the best stability in the leaves under low temperature stress.
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