Functional Study of MrSPL4 Gene in Response to Drought and Low Temperature Stress in Chinese Bayberry
To investigate the function of MrSPL4 in response to drought and low temperature stress in Chinese bayberry,the cis-acting element of MrSPL4 promoter and treating MrSPL4-OE transgenic tobacco T2 generation strain with drought and low temperature stress were analyzed,observing its seed germination rate and plant phenotype,and measuring plant physiological indexes to clarify the function of MrSPL4.The results showed that the promoter region of MrSPL4 contains cis-acting elements for drought induction,low temperature response,defense and stress response,and the other important cis-elements.Compared with wild-type tobacco(WT),under drought stress,MrSPL4-OE transgenic tobacco seed germination rate was significantly reduced.There was no significant difference in the reduction of plant water loss and wilting between WT and MrSPL4-OE.Except for a significant decrease in H2O2 content,there was no significant difference in other indicators.Transfer to normal conditions after low temperature stress,the germination rate of seeds significantly increased.Plant greening and wilting were reduced.SOD and POD enzyme activities increased significantly,while the content of MDA,H2O2,proline,and soluble sugar decreased significantly.The results indicated that MrSPL4 positively regulates the response process of tobacco to low temperature stress,improves its ability to tolerate low temperature,but inhibits the germination rate of tobacco seeds under drought stress.
Chinese bayberrySPL geneseed germinationdrought stresslow-temperature stress
NETL
NSTL
万方数据
