Effects of Overexpression of CsEXPA8 on Growth and Canker Disease Resistance in'Wanjincheng'Orange
To clarify the biological function of the expansion protein gene CsEXPA8,the vectors which excessed and inhibited the expression level of CsEXPA8 were constructed and then introduced in'Wanj incheng'orange,respectively.The phenotype of transgenic plants was observed and their resistance to citrus canker was evaluated.The results showed that CsEXPA8 has tissue specific expression characteristics and its expression level in'Wanj incheng'orange could be induced by Xanthomonas citri pv.citri,and that overexpression of CsEXPA8 could promote the growth of leaf length and width,and spring shoot,but also increase the sensitivity of transgenic plants to citrus canker,and that inhibiting the expression level of CsEXPA8 reduced the leaf length and width,and spring shoot,and enhanced the resistance of transgenic plants against citrus canker.These results indicated that CsEXPA8 positively and negatively regulates growth and resistance to citrus canker of'Wanj incheng'orange,respectively.
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