CsEXPA8过表达对'晚锦橙'生长及溃疡病抗性的影响
Effects of Overexpression of CsEXPA8 on Growth and Canker Disease Resistance in'Wanjincheng'Orange
彭爱红 1张婧芸 2陈志毅 1苏娟 1何永睿 1姚利晓1
作者信息
- 1. 西南大学柑桔研究所,重庆 400712
- 2. 西安市经开第五学校,西安 710018
- 折叠
摘要
为了明确扩展蛋白基因CsEXPA8的生物学功能,通过构建超量表达和干扰表达CsEXPA8的载体转化易感溃疡病的柑橘品种'晚锦橙',对获得的转基因植株进行表型分析和对溃疡病抗性评价.结果表明,CsEXPA8在'晚锦橙'中具有组织表达特异性,并受溃疡病菌诱导表达;CsEXPA8的过表达促进了叶片的长度和宽度的增加以及春梢直径的增粗,提高了对溃疡病的敏感性;而干扰CsEXPA8的表达后,转基因植株的叶片长度、宽度和春梢直径变小,但对溃疡病的抗性增强.上述结果表明,CsEXPA8正向调节了'晚锦橙'的生长,负向调控了'晚锦橙'对溃疡病的抗性.
Abstract
To clarify the biological function of the expansion protein gene CsEXPA8,the vectors which excessed and inhibited the expression level of CsEXPA8 were constructed and then introduced in'Wanj incheng'orange,respectively.The phenotype of transgenic plants was observed and their resistance to citrus canker was evaluated.The results showed that CsEXPA8 has tissue specific expression characteristics and its expression level in'Wanj incheng'orange could be induced by Xanthomonas citri pv.citri,and that overexpression of CsEXPA8 could promote the growth of leaf length and width,and spring shoot,but also increase the sensitivity of transgenic plants to citrus canker,and that inhibiting the expression level of CsEXPA8 reduced the leaf length and width,and spring shoot,and enhanced the resistance of transgenic plants against citrus canker.These results indicated that CsEXPA8 positively and negatively regulates growth and resistance to citrus canker of'Wanj incheng'orange,respectively.
关键词
柑橘/扩展蛋白/CsEXPA8/植物生长/柑橘溃疡病/抗性Key words
citrus/expansins/CsEXPA8/plant growth/citrus canker/resistance引用本文复制引用
基金项目
西南大学博士启动基金(SWU221004)
现代农业产业技术体系建设专项(CARS-26)
出版年
2024
NETL
NSTL
万方数据