Cloning of RhRNF185-like Gene from Rose and Its Effect on Petal Senescence
In order to explore the senescence mechanism of rose petals and provide gene reserve for the creation of anti-senescence rose varieties through molecular breeding and other means,a RhRNF185-like gene belonging to E3 ubiquitin ligase RING domain family were screened by transcriptome sequencing using cut rose'Carola'as the test material in this study.The results of qRT-PCR showed that the expression level of RhRNF185-like in the rose petals was significantly increased in the middle and late stage of rose flower opening,indicating that RhRNF185-like expression might affect the senescence of rose petals.Transient overexpression of RhRNF185-like in tobacco mesophyll cells showed that the GFP signal of RhRNF185-like coincided with the nuclear signal of DAPI through Confocal microscope,indicating that RhRNF185-like was located in the nucleus.Virus-induced gene silencing technique(VIGS)was used to silence RhRNF185-like gene in rose petals.It was found that the senescence degree of petals with RhRNF185-like gene silencing was significantly greater than that of TRV control group.In addition,compared with TRV control group,the relative expression level of senescence marker gene RhSAG12,ion permeability and fading rate of rose petals were significantly increased in the RhRNF185-like gene silencing group,indicating that silencing RhRNF185-like promoted the senescence of rose petals.These results indicated that RhRNF185-like affected the senescence of rose petals in an antagonistic regulatory manner.
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