Analysis of the Difference of Flavonoids in Dendropanax dentiger Leaves with Different Drying Processes Based on Untargeted Metabolomics
Ultrahigh performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was used to detect the flavonoids accumulated in leaves of Dendropanax dentiger under three drying methods(freeze-drying,hot-air drying and microwave drying).Principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA)were applied to identify the differential metabolites.The results indicated that 204 flavonoid compounds were identified,dominated by 53 flavonoids,37 flavonols and 24 isoflavones.A total of 80 differential compounds were screened,among which 69 different metabolites were found between hot-air drying and freeze-drying,67 were found between hot-air drying and microwave drying,and 45 were found between microwave drying and freeze-drying.The metabolites of these differences were mainly involved in the biosynthesis of secondary metabolites.The contribution rate of different substances to the characterization of different drying methods was obvious.Through the study of up-regulation and down-regulation of differential metabolites,it was found that the flavonoid components in the leaves could be retained better by freeze-drying,followed by microwave drying.
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