Studies on the Mechanism of α-Momordicin Gene Enhancing Tomato Resistance to Tobacco Mosaic Virus
To explore the mechanism of α-momordicin(αMMC)resistance to tobacco mosaic virus(TMV),the physiological parameters,virus accumulation,and the transcript levels of pathogenesis-related protein(PR)gene in αMMC transgenic plants and wild-type plants under TMV inoculation were analyzed using the'tomato-TMV'interaction system.Subcellular localization and phenotypic analysis showed that αMMC was a type Ⅰ ribosome-inactivating protein and localized in the cytoplasm.Furthermore,αMMC could inhibit virus accumulation in the host and alleviate the disease symptoms in tomato,positively the plant's defense system.Oxidative damage and enzyme activity assays showed that TMV infection caused an outbreak of reactive oxygen species,and αMMC could enhance the activities of antioxidant enzyme to remove excessive reactive oxygen species,resulting in less oxidative damage.In addition,real-time quantitative PCR results indicated that αMMC could specifically induce the expression level of defense-related genes PR1,PR4,PR6 and PRB1-3 to improve host resistance to TMV infection.
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