Biocontrol Efficacy and Mechanisms of Streptomyces purpeofuscus CC2-6 Against Chinese Cabbage Clubroot Caused by Plasmodiophora brassicae
In order to screen out biocontrol strains that can effectively inhibit Plasmodiophora brassicae,Fusarium oxysporum was used as the indicator fungus,and the strain CC2-6 was screened by plate confrontation method.The inhibitory rate was 50.03％.The fermentation broth of strain CC2-6 could inhibit the germination of resting spores of P.brassicae and the infection of P.brassicae from root hairs,and and the control efficacy of Chinese cabbage clubroot was 53.20％in pot trials.In addition,CC2-6 had inhibitory effects on nine pathogens including Pestalotiopsis nicotiae,and its inhibition rate rangeed more than 47.20％-76.19％,indicating that CC2-6 had broad-spectrum bacteriostatic activity.The strain CC2-6 was preliminarily identified as Streptomyces purpeofuscus by morphological observation and 16S rDNA gene sequence analysis.The strain CC2-6 could produce protease,cellulase and chitinase,and its fermentation broth could induce the increase of PPO,POD and PAL activities,the increase of SA and JA contents and the decrease of ET content in Chinese cabbage.The results of GC-TOF/LC-QTOF/QE non-target metabolomics showed that the fermentation broth of strain CC2-6 contained antagnostic active substances such as hygromycin B,camptothecin,2-phenylethanol and citric acid,indicating that CC2-6 might inhibit the infection of P brassicae by metabolizing these compounds.
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