Evaluation of Fruit Segmental Membrane Development and Mastication in Two Citrus Triploids
To investigate the reason for the fruit mastication difference between citrus triploid progeny with their diploid parents,two triploid hybrids derived from the crosses with Bendizao tangerine and Red tangerine as female parent were selected as materials to investigate the effect of segmental membrane structure and its components on the mastication of triploid fruits comparing with their diploid parents.In this study,using the two diploid parents as the control,the fruit mastication of the two triploid hybrids was firstly determined with texture analyzer at mature stage.It showed that the hardness and shear force of the segmental membrane of the two triploid progenies were significantly higher than that of their diploid parents,indicating that the segmental membranes of the two triploid hybrids might thicker than that of their corresponding diploid parents.It showed that the thickness and cell layer number of the segmental membranes of the two triploid hybrids were significantly higher than that of their corresponding diploid parents at the same developmental stage started from 120 days after flowering(DAF).The ultrastructure of the segmental membrane showed that the cell walls in the two triploid hybrids were thicker and the cellulose microfibrils were denser than those of their diploid parents.The content of water-soluble pectin,protopectin,cellulose and hemicellulose in the segment membrane of the two triploid hybrids were significantly higher than that of their diploid parents,while the lignin content was significantly lower at some developmental stage.In conclusion,segmental membrane thickness,water-soluble pectin,protopectin,cellulose and hemicellulose contents are important factors affecting the mastication of some citrus triploid hybrids,providing some useful evaluation indexes to screen superior triploid lines with good fruit mastication quickly from a large-scale triploid population.
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