Preliminary Study on the Mechanism of DXR Regulating Chloroplast Development Flower Color and Fruit Coloring in Tomato
In the growth and development of plants,carotenoids play a crucial role in leaf,flower,and fruit pigmentation,serving as the primary pigment compounds during fruit maturation.The l-deoxyd-D-xylose-5-phosphate reduction isomerase gene(DXR)is involved in the synthesis of carotenoids.The SlDXR gene editing and overexpression lines of tomato were constructed.It was found that gene editing lines exhibited yellow or even white leaves,the chlorophyll content and maximum photoenergy conversion efficiency of PS Ⅱ decreased significantly,the chloroplast development was affected,and the number of thylakoids was reduced.Meanwhile,the petal edge of gene editing lines turned white and the fruit color changed to orange or even yellow in the red ripening stage.The content of lycopene,flavonoid and other pigments in these fruits decreased to different degrees.There was no significant difference between the overexpression linesand the wild type(the control),however,the soluble solids content was significantly increased.Transcriptome sequencing showed that SlDXR was related to flavonoid biosynthesis,carotenoid biosynthesis and other pathways.These findings suggested that SlDXR could affect the coloring of tomato by regulating the synthesis of terpenoids.
tomatoDXRgene editingchlorophylllycopenefruit color
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