Cloning and Functional Verification of Linalool Synthase Gene PsTPS14 in Tree Peony'High Noon'
The linalool synthase gene PsTPS14 was cloned from the aromatic tree peony'High Noon'based on the transcriptome sequencing.The complete CDS sequence of this gene was 903 bp in length encoding 300 amino acids.The analysis of the amino acids sequence showed that the protein has one conservative domain(Terpene_cyclase_plant_Cl).The expression level of PsTPS14 was the highest in half opening stage of petals in tree peony'High Noon',consistent with the release pattern of floral volatiles.Subcellular localization analysis showed that PsTPS14 was mainly localized in the chloroplast.The overexpression vector of PsTPS14 was injected into tobacco leaves and the petals of cut flowers of'Fengdan',a light scent tree peony cultivar,by using the transient expression method.The release of linalool in tobacco leaves and'Fengdan'petals could be detected by GC-MS method.At the same time,the expression level of PsTPS14 was higher in tobacco leaves and'Fengdan'petals,and the content and activity of linalool synthase were significantly increased.This study showed that PsTPS14 regulated the synthesis of linalool in plants.
tree peonylinaloollinalool synthase genegene cloningfunctional verification
NETL
NSTL
万方数据
