The Mechanism of Heat Stress Response and the Exploration of Functional Genes in Rosa chinensis'Angela'
In this study,high temperature experiment was conducted and phylogenetic index were analyzed using Rosa chinensis'Angela''Renyue'and'Xianjing'as test materials.The results showed that'Angela'exhibited the highest heat resistance among the three varieties.Comparative transcriptome analysis,co-expression network analysis,protein-protein interaction network analysis and phylogenetic analysis revealed that the improvement of heat tolerance in'Angela'is associated with the overexpression of heat shock transcription factors(HSFs)and heat shock proteins(HSPs).Notably,the expression level of HSP gene RcHSP18.1 significantly increased with prolonged heat stress duration.Furthermore,by silencing the homologous gene NtHSP18.1 in tobacco,the heat resistance of tobacco was significantly reduced,confirming the essential role of HSP18.1 in heat stress tolerance.Overexpressing of RcHSP18.1 through gene bombardment and silencing RcHSP18.1 gene by virus induced gene silencing technique have both confirmed the significant role the gene RcHSP18.1 in enhancing heat stress resistance in'Angela'.
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