月季'安吉拉'热胁迫反应机制及功能基因的挖掘
The Mechanism of Heat Stress Response and the Exploration of Functional Genes in Rosa chinensis'Angela'
杨碧楠 1李博文 1杨振宇 1徐奕芃 1阎韵清 1娄玉霞 1奉树成 2明凤1
作者信息
- 1. 上海师范大学生命科学学院,植物种质资源开发协同创新中心,上海 200234
- 2. 上海市绿化管理指导站,上海 200020
- 折叠
摘要
以月季品种'安吉拉''韧月''仙境'为材料,模拟高温处理(46 ℃,4 h),通过表型以及生理指标分析,明确'安吉拉'对热胁迫的抵御能力最强.通过转录组、共表达、蛋白互作网络以及多态性分析发现,'安吉拉'较高的热胁迫抵御能力与热激蛋白(heat shock protein,HSP)以及热激转录因子(HSF)家族成员基因的超量表达有关.其中月季热激蛋白基因RcHSP18.1的表达水平受高温诱导提高最为显著.在烟草中瞬时沉默HSP18.1后发现,HSP18.1蛋白的表达能够显著提高宿主热胁迫的抵御能力.采用基因枪法对'安吉拉'月季愈伤组织进行RcHSP18.1过表达以及对植株进行病毒诱导基因沉默,均验证RcHSP18.1对抵御热胁迫提高发挥了重要作用.
Abstract
In this study,high temperature experiment was conducted and phylogenetic index were analyzed using Rosa chinensis'Angela''Renyue'and'Xianjing'as test materials.The results showed that'Angela'exhibited the highest heat resistance among the three varieties.Comparative transcriptome analysis,co-expression network analysis,protein-protein interaction network analysis and phylogenetic analysis revealed that the improvement of heat tolerance in'Angela'is associated with the overexpression of heat shock transcription factors(HSFs)and heat shock proteins(HSPs).Notably,the expression level of HSP gene RcHSP18.1 significantly increased with prolonged heat stress duration.Furthermore,by silencing the homologous gene NtHSP18.1 in tobacco,the heat resistance of tobacco was significantly reduced,confirming the essential role of HSP18.1 in heat stress tolerance.Overexpressing of RcHSP18.1 through gene bombardment and silencing RcHSP18.1 gene by virus induced gene silencing technique have both confirmed the significant role the gene RcHSP18.1 in enhancing heat stress resistance in'Angela'.
关键词
月季/热胁迫/基因/功能Key words
rose/heat stress/gene/function引用本文复制引用
基金项目
上海市科技兴农项目(2022-02-08-00-12-F01146)
上海市地方院校能力建设专项(20070502500)
上海植物种质资源工程技术研究中心项目(17DZ2252700)
出版年
2024
NETL
NSTL
万方数据