Analysis of Differential Genes in Response to Sucrose Feeding in Disc Florets of Chrysanthemum nankingense
To explore effects of sucrose feeding on the accumulation of total flavonoids in Chrysanthemum nankingense capitula and the underlying mechanism,Chrysanthemum nankingense,a unique wild diploid species of chrysanthemum in China,was used as the test material,and its detached capitula were fed with sucrose.The dynamic changes of total flavonoids and total phenols in disc and ray florets were determined at 0,12,36 and 60 h after sucrose feeding,and transcriptome sequencing of disc florets was performed.The results confirmed that sucrose feeding could increase the content of flavonoids and total phenols in disc florets within 24 h.However,in ray florets,these compounds changed slightly.A total of 9 135 differentially expressed genes(DEGs),which was also the maximum number of DEGs identified between treatments,were obtained by transcriptome analysis of disc florets sucrose-fed for 60 h and 0 h,while only 3 423 differentially expressed genes were obtained by transcriptome analysis of disc florets sucrose-fed for 60 h and no sucrose-fed for 60 h.These data indicate that the response degree of disc florets to sucrose increased with the increase of feeding time on the biochemistry level.KEGG analysis indicated that the flavonoid biosynthesis pathway was significantly enrichedand 32 flavonoid biosynthetic structural genes were differentially expressed.Correlation analysis showed that transcription factors MYBs,WRKYs and MADS-boxes had a strong co-expression relationship with flavonoid biosynthetic structural gene expression in disc florets in response to sucrose feeding.Nine DEGs were randomly selected for qRT-PCR,indicating that the transcriptome data were accurate.Spatio-temporal expression and subcellular localization of the encoding genes of two transcription factors were further analyzed to confirm the findings.Genes screened out from this study can offer potential targets for further efforts of metabolic engineering aiming to probe secondary metabolism in Chrysanthemum nankingense.
Chrysanthemum nankingenseresponse to sucroseflavonoid metabolismdifferentially expressed genesdisc florets
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