首页|蜡样芽孢杆菌挥发物对苹果轮纹病菌的拮抗活性

蜡样芽孢杆菌挥发物对苹果轮纹病菌的拮抗活性

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为了探索苹果轮纹病生物防治方法,分析了蜡样芽孢杆菌菌株G3-17挥发性有机化合物对苹果轮纹病菌的拮抗活性.平皿对扣试验显示,在前期研究基础上菌株G3-17不同浓度的挥发物均能显著抑制苹果轮纹病菌的生长.顶空固相微萃取—气相色谱—质谱联用分析显示,菌株G3-17主要分泌6种挥发性有机化合物,其中苯甲醛对苹果轮纹病菌的抑制效果最好,在浓度为125～625μL·L-1范围内抑制率为57.24％～100.00％.苯甲醛处理还能破坏苹果轮纹病菌细胞膜的完整性,并能诱导苹果果实病程相关蛋白基因(MdPR1和MdPR5)的表达.防效试验结果显示,苯甲醛处理能有效防治苹果轮纹病,在浓度为125、250和500 μL·L-1时的防效分别为39.80％、84.77％和90.67％.研究表明,蜡样芽孢杆菌菌株G3-17挥发物及主要成分苯甲醛对苹果轮纹病具有较好的防治潜力.
Antagonistic Activity of Volatile Organic Compounds of Bacillus cereus Strain G3-17 Against Apple Ring Rot
The purpose of this study is to analyze the antagonistic activity of volatile organic compounds(VOCs)of Bacillus cereus strain G3-17 against Botryosphaeria dothidea,and to explore the biological control methods of apple ring rot.The plate-to-plate experiment showed that the volatiles of Bacillus cereus strain G3-17 at different concentrations could significantly inhibit the growth of Botryosphaeria dothidea.Headspace solid-phase microextraction-gas chromatography-mass spectrometry analysis showed that the strain G3-17 mainly secreted six VOCs.Among them,benzaldehyde had the best inhibitory effect on Botryosphaeria dothidea,with an inhibition rate of 57.24％-100.00％in the concentration range of 125-625 μL·L-1.Further results also showed that benzaldehyde treatment also destroyed the integrity of the cell membrane of Botryosphaeria dothidea.In addition,benzaldehyde treatment could induce the expression of apple fruit pathogenesis-related protein genes(MdPR1 and MdPR5).Benzaldehyde treatment also could effectively control apple ring rot caused by Botryosphaeria dothidea,and the control effects were 39:80％,84.77％and 90.67％at the concentrations of 125,250 and 500 μL·L-1,respectively.In summary,the volatiles of Bacillus cereus strain G3-17 and its main VOCs have good control potential against apple ring rot.

Bacillus cereusapple ring rotvolatile organic compoundsbenzaldehydebiological control

覃艮红、袁洪波、王卓妮、史冰柯、范洋洋、王丽、张猛、涂洪涛、徐超、侯珲

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中国农业科学院郑州果树研究所,郑州 450009

河南农业大学植物保护学院,郑州 450009

中国农业科学院中原研究中心,河南新乡 453004

芽孢杆菌 苹果轮纹病 挥发性有机化合物 苯甲醛 生物防治

中央级公益性科研院所基本科研业务费专项中央级公益性科研院所基本科研业务费专项中国农科院科技创新工程项目河南省重点研发与推广专项

1610192023311ZGS202205CAAS-ASTIP-2016-RIP232102110052

2024

10.16420/j.issn.0513-353x.2023-0453

园艺学报
中国园艺学会 中国农业科学院蔬菜花卉研究所

园艺学报

CSTPCD北大核心
影响因子：1.127
ISSN：0513-353X
年,卷(期)：2024.51(6)
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