蜡样芽孢杆菌挥发物对苹果轮纹病菌的拮抗活性
Antagonistic Activity of Volatile Organic Compounds of Bacillus cereus Strain G3-17 Against Apple Ring Rot
覃艮红 1袁洪波 2王卓妮 3史冰柯 3范洋洋 3王丽 3张猛 4涂洪涛 2徐超 4侯珲2
作者信息
- 1. 中国农业科学院郑州果树研究所,郑州 450009;河南农业大学植物保护学院,郑州 450009
- 2. 中国农业科学院郑州果树研究所,郑州 450009;中国农业科学院中原研究中心,河南新乡 453004
- 3. 中国农业科学院郑州果树研究所,郑州 450009
- 4. 河南农业大学植物保护学院,郑州 450009
- 折叠
摘要
为了探索苹果轮纹病生物防治方法,分析了蜡样芽孢杆菌菌株G3-17挥发性有机化合物对苹果轮纹病菌的拮抗活性.平皿对扣试验显示,在前期研究基础上菌株G3-17不同浓度的挥发物均能显著抑制苹果轮纹病菌的生长.顶空固相微萃取—气相色谱—质谱联用分析显示,菌株G3-17主要分泌6种挥发性有机化合物,其中苯甲醛对苹果轮纹病菌的抑制效果最好,在浓度为125～625μL·L-1范围内抑制率为57.24％～100.00％.苯甲醛处理还能破坏苹果轮纹病菌细胞膜的完整性,并能诱导苹果果实病程相关蛋白基因(MdPR1和MdPR5)的表达.防效试验结果显示,苯甲醛处理能有效防治苹果轮纹病,在浓度为125、250和500 μL·L-1时的防效分别为39.80％、84.77％和90.67％.研究表明,蜡样芽孢杆菌菌株G3-17挥发物及主要成分苯甲醛对苹果轮纹病具有较好的防治潜力.
Abstract
The purpose of this study is to analyze the antagonistic activity of volatile organic compounds(VOCs)of Bacillus cereus strain G3-17 against Botryosphaeria dothidea,and to explore the biological control methods of apple ring rot.The plate-to-plate experiment showed that the volatiles of Bacillus cereus strain G3-17 at different concentrations could significantly inhibit the growth of Botryosphaeria dothidea.Headspace solid-phase microextraction-gas chromatography-mass spectrometry analysis showed that the strain G3-17 mainly secreted six VOCs.Among them,benzaldehyde had the best inhibitory effect on Botryosphaeria dothidea,with an inhibition rate of 57.24％-100.00％in the concentration range of 125-625 μL·L-1.Further results also showed that benzaldehyde treatment also destroyed the integrity of the cell membrane of Botryosphaeria dothidea.In addition,benzaldehyde treatment could induce the expression of apple fruit pathogenesis-related protein genes(MdPR1 and MdPR5).Benzaldehyde treatment also could effectively control apple ring rot caused by Botryosphaeria dothidea,and the control effects were 39:80％,84.77％and 90.67％at the concentrations of 125,250 and 500 μL·L-1,respectively.In summary,the volatiles of Bacillus cereus strain G3-17 and its main VOCs have good control potential against apple ring rot.
关键词
芽孢杆菌/苹果轮纹病/挥发性有机化合物/苯甲醛/生物防治Key words
Bacillus cereus/apple ring rot/volatile organic compounds/benzaldehyde/biological control引用本文复制引用
基金项目
中央级公益性科研院所基本科研业务费专项(1610192023311)
中央级公益性科研院所基本科研业务费专项(ZGS202205)
中国农科院科技创新工程项目(CAAS-ASTIP-2016-RIP)
河南省重点研发与推广专项(232102110052)
出版年
2024
NETL
NSTL
万方数据