拮抗番茄灰霉病的贝莱斯芽孢杆菌XDY66基因组分析
Genome Analysis of Bacillus velezensis XDY66,an Antagonist of Tomato Botrytis cinerea
张文静 1徐大勇 1吴倩琳 1杨佛 1信丙越 1曾昕 1李峰1
作者信息
- 1. 淮北师范大学生命科学学院,资源植物生物学安徽省重点实验室,安徽淮北 235000
- 折叠
摘要
从番茄根际土壤中分离筛选到1株对灰葡萄孢(Botrytis cinerea)有强抑制作用的贝莱斯芽孢杆菌(Bacillus velezensis)XDY66.离体测定及活体试验表明XDY66对灰葡萄孢、辣椒疫霉、番茄枯萎病菌均有较强的拮抗作用;测序结果表明XDY66基因组全长3.964 552 Mb,包含1个环状染色体(大小 3.956 418 Mb,GC 含量 46.53％,NCBI 登录号 CP130608)和 1 个质粒(大小 8 134 bp,GC 含量 40.31％,NCBI登录号CP130609);比较基因组分析表明,XDY66含有176个特有基因,与Bacillus velezensis SQR9具有更近的亲缘关系,其次为Bacillus velezensis FZB42;AntiSMASH分析结果显示,XDY66基因组含有13个次生代谢产物合成基因簇,其中包括合成脂肽类(surfactin、fengycin)、铁载体类(bacillibactin)、聚酮类(bacillaene、macrolactin、difficidin)和二肽化合物(bacilysin)等多种化合物的基因簇.本研究的结果表明,Bacillus velezensis XDY66具有防治番茄灰霉病等作物病原真菌的生防潜力.
Abstract
Bacillus velezensis XDY66 was isolated and screened from rhizosphere soil from tomato,which showed strong inhibition to Botrytis cinerea.The antagonistic activity of Bacillus velezensis XDY66 against Botrytis cinerea was evaluated in vitro and in vivo.The results showed that the total length of Bacillus velezensis XDY66 genome is 3.964 552 Mb,which contained a circular chromosome(3.956 418 Mb,GC content 46.53％,NCBI accession number CP130608)andaplasmid(8 134 bp,GC content was 40.31％,NCBI accession number CP130609).Comparative genomic analysis showed that Bacillus velezensis XDY66 contained 176 unique genes.In addition,Bacillus velezensis XDY66 was closely related to Bacillus velezensis SQR9,next is Bacillus velezensis FZB42.AntiSMASH analysis showed that the Bacillus velezensis XDY66 genome contained 13 gene clusters for secondary metabolites synthesis,including lipopeptides(surfactin,fengycin),siderophore(bacillibactin),polyketides(bacillaene,macrolactin,difficidin),and dipeptide compound(bacilysin)etc.These results showed that Bacillus velezensis XDY66 could be potentially applied in bio-controlling of crop pathogenic fungi,such as tomato Botrytis cinerea.
关键词
番茄/灰葡萄孢/贝莱斯芽孢杆菌/全基因组测序/比较基因组分析Key words
tomato/Botrytis cinerea/Bacillus velezensis/whole genome sequencing/comparative genomic analysis引用本文复制引用
基金项目
安徽省自然科学基金(1808085MC74)
安徽省高等学校自然科学研究重点项目(KJ2018A0676)
安徽省高等学校优秀拔尖人才培育项目(gxgnfx2021118)
安徽省高等学校自然科学现代农业技术创新团队项目(2023AH010045)
出版年
2024
NETL
NSTL
万方数据