Establishment of Tissue Culture and Virus Eliminating Technology for Shallot
The bulb tip of shallot was used as explants to obtain tissue culture seedlings by direct induced clustered shoots and callus-induced differentiation.The disinfection treatment,plant growth regulator ratio of medium and the size of bulb tip were optimized,and the virus-free effect of shallot latent virus(SLV)and onion yellow dwarf virus(OYDV)in tissue culture seedlings was tested by ELISA.The results showed that the bulb tip explants were soaked in 75％ethanol for 30 s and 2％sodium hypochlorite solution for 15 min.The pollution rate was 6.7％and the survival ratewas 93.3％.The optimal bulb tip induction medium was MS+0.5 mg·L-1 NAA+1.0 mg·L-16-BA,with the bud induction rate of 85.6％and the average number of differentiated buds was 3.3.The optimal callus medium was MS+0.4 mg·L-1 NAA+0.5 mg·L-12,4-D and the callus rate was 71.7％.The optimal bud-forming medium was MS+0.4 mg·L-1NAA+1.0 mg·L-1 6-BA,with the bud-forming rate was 82.2％and the average number of seedlings was 9.23.The rooting requirement could be satisfied in 1/2MS medium without plant growth regulator ratio,and the rooting rate could reach 100％.The shallot virus-free seedlings were obtained by stripping the bulb tips of 0.3-0.4 mm,with the virus-free rate of 92％.The survival rate of tissue culture seedlings was 90％after transplanting.
shallotbulb tip inductioncallus differentiationvirus-free seedling
NETL
NSTL
万方数据
