The bulb tip of shallot was used as explants to obtain tissue culture seedlings by direct induced clustered shoots and callus-induced differentiation.The disinfection treatment,plant growth regulator ratio of medium and the size of bulb tip were optimized,and the virus-free effect of shallot latent virus(SLV)and onion yellow dwarf virus(OYDV)in tissue culture seedlings was tested by ELISA.The results showed that the bulb tip explants were soaked in 75％ethanol for 30 s and 2％sodium hypochlorite solution for 15 min.The pollution rate was 6.7％and the survival ratewas 93.3％.The optimal bulb tip induction medium was MS+0.5 mg·L-1 NAA+1.0 mg·L-16-BA,with the bud induction rate of 85.6％and the average number of differentiated buds was 3.3.The optimal callus medium was MS+0.4 mg·L-1 NAA+0.5 mg·L-12,4-D and the callus rate was 71.7％.The optimal bud-forming medium was MS+0.4 mg·L-1NAA+1.0 mg·L-1 6-BA,with the bud-forming rate was 82.2％and the average number of seedlings was 9.23.The rooting requirement could be satisfied in 1/2MS medium without plant growth regulator ratio,and the rooting rate could reach 100％.The shallot virus-free seedlings were obtained by stripping the bulb tips of 0.3-0.4 mm,with the virus-free rate of 92％.The survival rate of tissue culture seedlings was 90％after transplanting.
shallotbulb tip inductioncallus differentiationvirus-free seedling
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