植物VIGS载体构建策略研究与应用进展
Research Advances of Strategies to Engineer VIGS Vectors and Its Application in Plants
任恒泽 1李丹莹 1余亚婷 1吕务云 1郝心愿 2王新超 2王玉春1
作者信息
- 1. 浙江农林大学茶学与茶文化学院,杭州 311300
- 2. 中国农业科学院茶叶研究所,国家茶树改良中心,农业农村部特种经济动植物生物学与遗传育种重点实验室,杭州 310008
- 折叠
摘要
病毒诱导的基因沉默(virus-induced gene silencing,VIGS)是一种基于植物抗病毒机制开发的用于基因功能研究的反向遗传学技术.该技术不依赖植物遗传转化体系,具有周期短、操作简单高效、高通量的特点,被广泛应用于植物生长发育、信号转导、代谢途径和抗逆机制等方面的研究.本文中综述了植物VIGS作用机理、应用和存在的问题,重点讨论了载体构建策略和影响因素等方面的内容,旨在为该技术的进一步发展和应用提供参考.
Abstract
Virus-induced gene silencing(VIGS),a reverse genetic technique based on plant antiviral mechanism,has been widely used in the study of plant growth and development,signal transduction,metabolic pathways and stress resistance due to its advantages of independent of plant genetic transformation system,time-saving,easy and efficient operation,and high-throughput.In this paper,the mechanism,application and problems of VIGS were reviewed,and the vector construction strategies and influencing factors were discussed emphatically,aiming to provide reference for the further development and application of VIGS technology.
关键词
病毒诱导的基因沉默/基因功能/病毒载体/沉默载体构建策略Key words
virus-induced gene silencing/gene function/viral vector/silencing vector construction strategy引用本文复制引用
基金项目
浙江农林大学科研发展基金项目(2023FR008)
浙江省教育厅一般科研项目(Y202353277)
浙江省农业新品种选育重大专项(2021C02067-7)
出版年
2024
NETL
NSTL
万方数据