Research Advances of Strategies to Engineer VIGS Vectors and Its Application in Plants
Virus-induced gene silencing(VIGS),a reverse genetic technique based on plant antiviral mechanism,has been widely used in the study of plant growth and development,signal transduction,metabolic pathways and stress resistance due to its advantages of independent of plant genetic transformation system,time-saving,easy and efficient operation,and high-throughput.In this paper,the mechanism,application and problems of VIGS were reviewed,and the vector construction strategies and influencing factors were discussed emphatically,aiming to provide reference for the further development and application of VIGS technology.
virus-induced gene silencinggene functionviral vectorsilencing vector construction strategy
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