Screening of Ziziphus jujuba'Hupingzao'Fruit Coloring Substances and Their Related Genes
The white ripening,semi-red and whole red fruits of Ziziphus jujuba'Hupingzao'as materials,the contents of the main pigment substances in the peel were determined and transcriptome sequencing was carried out to elaborate the changes of coloring genes in the peel of jujube at the molecular level.The results showed that after the white ripening period,the anthocyanin content of jujube peel began to decrease significantly.In the three periods,the flavonoid content of jujube peel decreased significantly.The chlorophyll content did not change much from the white maturity stage to the semi-red stage,and decreased significantly at the full red stage.The content of carotenoids was relatively stable in the veraison stage.Flavonoids,anthocyanins,carotenoids and chlorophyll are the coloring substances of jujube peel,and the anthocyanins are divided into six types:delphinidin,cyanidin,petunidin,malvidin,peonidinand pelargonidin.According to the GO and KEGG enrichment analysis in transcriptome sequencing,the number of differential genes in flavonoids,anthocyanins,flavonoids and flavonols,carotenoids and porphyrin metabolic pathways were 35,22,6,30 and 38,respectively.Among them,the white anthocyanidin reductase gene(LOC107429483),transcription factor gene MYC2(LOC107430586)and dihydroflavonol 4-reductase gene(LOC107420289)played a major role in the coloring of jujube peel,and β-carotene hydroxylase gene(LOC107414761)was the key gene affecting carotenoid synthesis.The chloroplast A oxygenase gene(LOC107410684)in porphyrin metabolism is a key gene affecting total chlorophyll content.
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