Screening of Kiwifruit Canker Resistance-Related Genes Based on the Transcriptome Sequencing Analysis of Hybrids from Actinidia chinensis
Kiwifruit bacterial canker is a devastating disease in the kiwifruit industry,causing huge losses to the industry.Great differences in resistance are found in materials with different genetic background.To study the early response of kiwifruit to canker and to explore the genes related to resistance,transcriptome sequencing and comparative analysis was performed on the resistant'21-2-4'and the susceptible'19-3-11'in the offspring of Actinidia chinensis'Jinnong'× male after 6 h and 24 h inoculation with Pseudomonas syringae pv.actinidiae(Psa).The results showed that the defense of'21-2-4'and'19-3-11'against Psa mainly focused on 24 h and 6 h respectively.KEGG analysis showed that DEGs were mainly enriched in the primary and secondary metabolic pathways such as plant-pathogen interaction,phenylpropanoid biosynthesis,starch and sucrose metabolism.Gene expression patterns in resistant and susceptible materials were compared,and possible candidate genes in disease-esistant pathways such as plant-pathogen interaction,plant hormone signal transduction,and phenylpropane biosynthesis were screened.By using the resistant'Jinnong'and susceptible'Hongsheng',the genes screened were verified by qRT-PCR,and then PR1(Acc06864/Acc06865/Acc06866)and FLS2(Acc06484)were selected as candidate genes for further study.
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