Transcription Metabolic Mechanism of Humic Acid Biodegradable Plastic Film to Improving the Fruit Quality of Tomato in the Greenhouse
Humic acid biodegradable plastic film is a new type of biodegradable plastic film that has received widespread attention due to its significant improvement in the soil quality of facility cultivation and the increase in tomato quality.To investigate the effects and regulatory mechanisms of humic acid biodegradable plastic film on the quality and nutritional components of tomato fruits,this study used facility tomato'Tiancuicui'as the experimental material,flavor sensory evaluation was conducted on mature tomato fruits treated with PBAT/PLA ordinary degradable plastic film(PT)and humic acid biodegradable plastic film(FZS),and the content of soluble sugars,lycopene and other nutrients were measured.PCA,OPLS-DA,KEGG and other analysis methods were used to jointly analyze the transcriptome and GC/LC-MS non targeted metabolomic of ripe fruits.The results showed that FZS treatment significantly reduced the number of thin-walled cells by 28.10％,thickness of epidermis and stratum corneum in tomato fruit by 24.28％and 43.99％,respectively,promoting tomato fruit ripening,increasing soluble sugar and sugar acid ratio,reducing fruit acidity and hardness,and improving fruit quality;transcriptome analysis identified 500 differentially expressed genes,mainly enriched in pathways such as carbohydrate metabolism,glycolysis,and cell wall structure;metabolomic analysis identified 143 differential metabolites,mainly enriched in pathways including butyric acid metabolism,lysine degradation,and ABC transporters;transcription metabolism joint analysis revealed that the impact of FZS on tomato fruit flavor quality is positively regulated by the carbohydrate/energy metabolism pathway,amino acid and hormone transport;the carbohydrate metabolism pathway involved in glucose-6-phosphate,fructose-6-phosphate,and galactose are the main regulatory pathways.
NETL
NSTL
万方数据
